【个人简介】
姓名：郭忠建
性别：男
职称：副研究员
E-mail:gzh762677@ujs.edu.cn
【学习经历】
1995-1999年，郑州工程学院粮油储藏系，学士学位；
1999-2002年，河南工业大学粮油食品学院，硕士学位；
2002-2005年，浙江大学农业与生物技术学院，博士学位。
【工作经历】
2005-现，江苏大学生命科学研究院，助理研究员、副研究员；
2011-2012年，美国路易斯安那州立大学动物医学学院，博士后。
【研究领域】
1、酶制剂
酶催化是生物制造的核心内容，其高催化活性、高稳定性及循环利用是构建高效低成本酶催化工艺的关键。我们以芽孢杆菌、酵母菌、乳杆菌等菌种，表达或展示固定化酶、计算模拟、理性设计、连接肽优化、拓展应用，用于饲料、造纸、化工、食品、医药、环境等行业。
2、新型高效低成本的微生物饲料研究及产业化
应用微生物及酶制剂，包括菌种的筛选、CRISPR-Cas9基因编辑、合成生物学和生物饲料，菌酶发酵工艺、中试、产业化及实际投入使用的效果研究，涉及的微生物有芽孢杆菌、酵母菌、乳酸菌、双岐杆菌、霉菌等。
【结题项目】
江苏省教育厅自然科学基金1项（BmNPV泛素蛋白修饰的蛋白质质谱鉴定，批准号：07KJB180013，2007年7月-2009年12月），主持。
江苏大学高级人才基金1项（杆状病毒泛素基因的功能，批准号：05JDG048），主持。
中国博士后科学基金1项（家蚕与BmNPV在泛素通道上的敌我识别研究，批准号：20100471387），主持。
国家自然科学基金面上项目(名称：家蚕核型多角体病毒基因组复制受宿主分子伴侣HSC/HSP70调控的机制，批准号：31370184，起止年月：2014年1月-2017年12月)，经费总额：80万，主持。
江苏省自然科学基金面上项目（名称：分子伴侣HSC/HSP70对家蚕杆状病毒复制的调控机制，批准号：BK2012692，起止年月：2012年7月-2015年6月），经费总额：10万，第一参与人。
国家自然科学基金面上项目(名称：分子定向固定化NADH氧化酶的研究，批准号：21376110，起止年月：2014年1月-2017年12月)，经费总额：80万，第一参与人。
国家自然科学基金面上项目(名称：BmNPV多角体蛋白“操纵”细胞自噬以利于多角体形成的分子机制，批准号：31770174，起止年月：2018年1月-2021年12月)，经费总额：65万，主持。
【在研项目】
新疆生产建设兵团科技发展专项资金项目（产L-赖氨酸植物乳杆菌工程菌的构建及其对肉羊肠道内源合成的作用与机制研究），2026-2028年度。合作单位。
【发表论文】
28、Wang XY, Chen HY, Chen HQ, Yu HW, Ye LD, Guo ZJ. 2026. Metabolic and synthetic biology strategies for enhancing single-cell protein production in Saccharomyces cerevisiae. Biotechnology Journal, 21(6): e70273.
27、Cai KT, Lu CX, Wu ZF, Tan JC, Chen HY, Wu P, Guo ZJ, Feng Y. 2026. Optimization of nitrogen removal and microbial communities in biofloc systems via carbon-to-nitrogen ratio regulation and Candida utilis. BMC Biotechnology, 26(1): 74.
26、Ullah M, Han JD, Zhu XM, Guo ZJ, Najumuddin, Naeem M, Andoh V, Guan GQ, Bogale A, Alyami EM, Alghamdi OA, Dawoud TM, Chen HY. 2026. Site-directed mutagenesis and semi-rational design to enhance chitinolytic activity of bacterial exochitinase. Microbial Cell Factories, 25(1): 164.
25、Li MM, Naeem M, Guo ZJ, Dong TC, Zhu XM, Hussain N, Ni Z, Chen HY. 2026. Synthetic biology for next generation lactic acid bacteria: engineering the future of food. Journal of Agricultural and Food Chemistry, 74(21), 15989-16014.
24、Ma Y, Zhang JT, Chen C, Wang MZ, Li HF, Deng YF, Zhou XR, Hu JH, Zhang, H, Li GH, Wang J, Guo ZJ. 2026. Thiamine ameliorates subacute ruminal acidosis-induced mastitis in goats and is associated with modulation of the NF-κB/NLRP3/CLOCK axis and rumen microbial homeostasis. Journal of Dairy Science, 109(5), 5112-5129.
23、Feng Y, Chen XH, Li ZY, Ni Z, Wu ZF, Guo ZJ, Sun FB, Chen HQ, Chen HY. 2026. Engineering a broad-spectrum glucose oxidase via substrate channel and linker design for enhanced lignocellulose bioconversion. Synthetic and Systems Biotechnology, 12, 218-228.
22、Lu CX, Cai KT, Chen XH, Wang Z, Chen HY, Wu P, Guo ZJ, Feng Y. 2026. Effects of fermented compound chinese herbal feed on gut microbiota, immune response, and disease resistance in Chinese soft-shelled turtle (Pelodiscus sinensis). Animals, 16(7), 1054.
21、Ni Z, Zhao J, Zhou HM, Jia HY, Gao EB, Guo ZJ, Hu ZY, Ma SS, Chen Y. 2026. Mechanistic investigation of the enhanced catalytic activity of B. sub lipase A mutant I157V and its application in biodiesel production. Enzyme and Microbial Technology, 194, 110798.
20、Shi N, Cheng D, Chen Y, Feng Y, Saeed M, Guo ZJ, Hu ZY, Chen HY. 2025. Improvement in lignin peroxidase oxidative stability via surface display by Bacillus subtilis. Biotechnology Letters, 47(4), 86.
19、Chen YZ, Li MM, Chen HY, Saeed M, Ni Z, Guo ZJ, Fang Z , Feng Y. 2025. Directed evolution modification of the keratinase KerD21 and application in feather powder fermented feed. Journal of the Science of Food and Agriculture, 105(14), 8171-8185.
18、Chen XH, Li ZY, Wu ZF, Feng Y, Chen Y, Ma Y, Guo ZJ, Chen HY. 2025. Spore display innovation technology for unlocking new substrates for glucose oxidase. Journal of the Science of Food and Agriculture, 105(13), 7112-7123.
17、Li ZY, Chen Y, Chen XH, Guo ZJ, Guan GQ, Feng Y, Chen HY. 2025. Modification and applications of glucose oxidase: optimization strategies and high-throughput screening technologies. World Journal of Microbiology and Biotechnology, 41(7), 266.
16、Xia YT, Zhao J, Saeed M, Hussain N, Chen XH, Guo ZJ, Yong YC, Chen HY. 2024. Molecular modification strategies of nitrilase for its potential application in agriculture. Journal of Agricultural and Food Chemistry, 72(27), 15106-15121.
15、Han JD, Ullah M, Andoh V, Khan MN, Feng Y, Guo ZJ, Chen HY. 2024. Engineering bacterial chitinases for industrial application: from protein engineering to bacterial strains mutation! A comprehensive review of physical, molecular, and computational approaches. Journal of Agricultural and Food Chemistry, 72(42), 23082-23096.
14、Wu ZF，Li PF，Chen Y，Chen XH，Feng Y，Guo ZJ，Zhu DC，Yong YC，Chen HY. 2024. Rational design for enhancing cellobiose dehydrogenase activity and its synergistic role in straw degradation. Journal of Agricultural and Food Chemistry, 72(44), 24620-24631.
13、Guo, Z.J., Yu, M.H., Dong, X.Y., Wang, W.L., Tian, T., Yu, X.Y., Tang, X.D. Protein composition analysis of polyhedra matrix of Bombyx mori nucleopolyhedrovirus (BmNPV) showed powerful capacity of polyhedra to encapsulate foreign proteins. Scientific Reports, 2017, 7: 8768. (SCI)
12、Guo, Z.J., Tao, L.X., Dong X.Y., Yu M.H., Tian T., Tang X.D. Characterization of aggregate/aggresome structures formed by polyhedrin of Bombyx mori nucleopolyhedrovirus. Scientific Reports, 2015, 5: 14601. (SCI)
11、Guo, Z.J., Zhu, Y.M., Li, G.H., Chen, K.P., Zhang, C.X., 2011. Ubiquitins of Bombyx mori nucleopolyhedrovirus and Helicoverpa armigera nucleopolyhedrovirus show distinct subcellular localization in infected cells. Acta Virologica 55, 101-106. (SCI)
10、Guo, Z.J., Wang, D.X., Yao, Q., Chen, K.P., Zhang, C.X., 2010. Identification of a novel functional nuclear localization signal in the protein encoded by open reading frame 47 of Bombyx mori nucleopolyhedrovirus. Archives of Virology 155, 1943-1950. (SCI)
9、Guo, Z.J., Qiu, L.H., An, S.H., Yao, Q., Park, E.Y., Chen, K.P., Zhang, C.X., 2010. Open reading frame 60 of the Bombyx mori nucleopolyhedrovirus plays a role in budded virus production. Virus Research 151, 185-191. (SCI)
8、Guo, Z.J., Qiu, L.H., Du, M.F., An, S.H., 2009. Characterization of ORF127 of Helicoverpa armigera nucleopolyhedrovirus. Acta Virologica 53, 247-253. (SCI)
7、Guo, Z.J., Ge, J.Q., Wang, D., Shao, Y.M., Tang, Q.Y., Zhang, C.X., 2006. Biological comparison of two genotypes of Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus. BioControl 51, 809- 820. (SCI)
6、Guo, Z.J., An, S.H., Wang, D., Liu, Y.H., Kumar, V.S., Zhang, C.X., 2005. Characterization of Ha29, a specific gene for Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus. Journal of Biochemistry and Molecular Biology 38, 354-359. (SCI)
5、Zhu, Y.M., Li, G.H., Yao, Q., Chen, K.P.,Guo, Z.J.*, 2010. Characterization of the open reading frame 35 of Bombyx mori Nucleopolyhedrovirus. International Journal of Industrial Entomology 21, 157-162.
4、Zhang, C.X., Ma, X.C.,Guo, Z.J., 2005. Comparison of the complete genome sequence between C1 and G4 isolates of the Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus. Virology 333, 190-199. (SCI)
3、An, S.H.,Guo, Z.J., Yin, X.M., 2006. Expression and immunocytochemical analysis of Autographa californica nucleopolyhedrovirus (AcMNPV) orf74 gene. Insect Science 13, 349-354.
2、Wang, D., An, S.H.,Guo, Z.J., Xu, H.J., Zhang, C.X., 2005. Characterization of Helicoverpa armigera nucleopolyhedrovirus orf33 that encodes a novel budded virion derived protein, BV-e31. Archives of Virology 150, 1505-1515. (SCI)
1、Du, M.F., Yin, X.M.,Guo, Z.J., Zhu, L.J., 2006. Characterization of a late gene, ORF60 from Bombyx mori nucleopolyhedrovirus. Journal of Biochemistry and Molecular Biology 39, 737-742. (SCI)
更新于2026年9月
